Production of sarcoma in rats with light green SF.

In 1937 Schiller (1) reported that the dye, light green SF, was carcinogenic for rats but not for mice. In his experiment, 7 rats (white, and spotted black and white) received, once or twice weekly, subcutaneous injections of 1 to 3 cc. of an aqueous 2 per cent, and uhlmately a 3 per cent, solution of the dye. When the animal's condition deteriorated, the injections were omitted or the volume was diminished. After about a year, 4 of the rats developed sarcomas at the site of injection. As Schiller's experiment was conducted with a small number of heterogeneous animals, and since no confirmation had appeared in print, it seemed desirable to repeat Schiller's experiments, using a sample of light green SF supplied by Coleman and Bell.